In this study, a nude mouse model was established to observe the in vivo antiproliferative and apoptotic effects of pomegranate peel polyphenols.
CONDUCTED BY: College of Pharmacy, Xinjiang Medical University, China; The First Teaching Hospital of Xingjiang Medical University, China; Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, China
PUBLISHED ON: Food Science and Biotechnology
Punica granatum possesses strong antitumor properties. In this study, a nude mouse model with a subcutaneous xenograft of human prostate cancer cells (PC-3) was established to observe the in vivo antiproliferative and apoptotic effects of pomegranate peel polyphenols. Levels of the cytokines tumor necrosis factor α (TNF-α) and vascular endothelial growth factor (VEGF) were detected by enzyme-linked immunosorbent assay. Quantitative analysis of ellagic acid, gallic acid, and punicalagin in active fraction was conducted using reverse phase high-performance liquid chromatography with a photo-diode array (HPLC/PDA). Pomegranate peel polyphenols decreased tumor volume and weight in tumor-bearing nude mice, and significantly increased the rate of apoptosis (p<0.05). In addition, pomegranate peel polyphenols increased TNF-α and decreased VEGF in the serum (p<0.05). Ellagic acid (201.3±3.544 mg/g), gallic acid (8.917±0.274 mg/g), and punicalagin (407.0±12.05 mg/g) were the main effectors of the anti-tumor activity.