CONDUCTED BY: Department of Pharmacology, K S Hegde Medical Academy, Mangalore;Department of OBG, K S Hegde Medical Academy, Mangalore; Department of Physiology, K S Hegde Medical Academy, Mangalore.
To explore the potential role of Punica granatum ethanolic extract (PGEE) in Cyclophosphamide (CP) induced testicular toxicity.
Healthy male Wistar rats were allotted to 4 groups (N=6, each) Group I: Control, Group II: Cyclophosphamide 15mg/kg twice a week, Group III: Punica granatum ethanolic extract 100mg/kg, Group IV: Cyclophosphamide and Punica granatum ethanolic extract for 28 days. At the end of the treatment period, organ weight, body weight, epididymal sperm count, motility, morphology, SOD, catalase, GSH, ACP & testosterone level in the testis were evaluated.
The Cyclophosphamide treated rats showed toxicity evidenced by decreased organ and body weight, decreased sperm quality and testosterone level also increase in MDA and decrease in antioxidants SOD, GSH indicating oxidative stress.
In contrast Punica granatum ethanolic extract co-treatment with Cyclophosphamide resulted in significant restoration of the above mentioned parameters.
These results indicate that Punica granatum ethanolic extract attenuates Cyclophosphamide induced testicular toxicity through its ROS scavenging activity.