WEB TITLE: Pomegranate peel extract on melanoma cell line
CONDUCTED BY: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
PUBLISED ON: Research in Pharmaceutical Sciences
In the present study possible effects of black pomegranate peel extract (PPE) on the B16F10 melanoma cells proliferation and Human Umbilical Vein Endothelial Cells (HUVECs) angiogenesis were investigated. pomegranate peel extract was added into the cell lines (B16F10 and HUVECs) media with different concentrations (10–450 µg/ml). After 48 h, the cell survival was measured by 3-(Dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Angiogenesis was investigated by matrigel assay (PPE (200, 300, 400 µg/ml)); HUVECs, vascular endothelial growth factor (VEGF) mRNA expression was detected by quantitative reverse transcriptase–polymerase chain reaction (QRT-PCR) assay.
VEGF concentration in culture medium of HUVECs was determined by enzyme-linked immunosorbent assay (ELISA). PPE had positive anti proliferative effect on melanoma cells in a dose-dependent manner, but not on HUVECs. The matrigel assay results indicated that PPE significantly inhibited length, size and junction of the tube like structures (P<0.05). VEGF mRNA expression and concentration levels in culture medium of pomegranate peel extract treated HUVECs reduced significantly in a concentration-dependent manner (P<0.05). Simultaneous inhibition of melanoma cell proliferation and angiogenesis proposed that, PPE can be a good candidate against melanoma development. Based on the results, PPE could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results
Simultaneous inhibition of melanoma cell proliferation and angiogenesis indicates that, pomegranate peel extract can be a good candidate against melanoma development. Based on the results of the present study, pomegranate peel extract could effectively suppress angiogenesis potentially through a VEGF dependent mechanism. Further studies are needed to confirm these results.