RESEARCH TITLE: Evaluation of Punica Granatum fruit peels extracts for its free radical scavenging and anti- inflamatory activity
WEB TITLE: Pomegranate Punica Granatum fruit peels extracts for its free radical scavenging and anti- inflamatory activity
COUNTRIES: India
CONDUCTED BY: Minal campus, Bhopal, India
PUBLISHED ON: International Journal of Pharmacy and Pharmaceutical Sciences
RESEARCH:
Abstract
Objective: To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethylacetate, aqueous, methanol:water and methanol extracts of Punica granatum fruit peels (Family: Lythraceae) by in vitro methods.
Methods: The free radical scavenging effect was studied using 1, 1‐Diphenyl‐2‐ Picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay. Anti‐inflammatory activity was evaluated by HRBC membrane stabilization assay.
Results: All the extracts of Punica granatum fruit peels exhibited significant free radical scavenging effect. The methanol extract exhibited maximum significant DPPH and nitric oxide radical scavenging activity with IC50 value of 24.43 and 45.56µg/ml and maximum stabilization (86.96%) of HRBC membrane at 80µg/ml among all the extracts of Punica granatum fruit peels.
Conclusion: Methanol as an extraction solvent was found to be the best in obtaining the extract of Punica granatum fruit peels rich in radical scavenging and anti-inflammatory phytoconsituents.
RESEACH SUMMARY:
To evaluate free radical scavenging and anti-inflammatory activity of petroleum ether, ethylacetate, aqueous, methanol: water and methanol extracts of Punica granatum fruit peels (Family: Lythraceae) by in vitro methods.All the extracts of Punica granatum fruit peels exhibited significant free radical scavenging effect. The methanol extract exhibited maximum significant DPPH and nitric oxide radical scavenging activity with IC50 value of 24.43 and 45.56µg/ml and maximum stabilization (86.96%) of HRBC membrane at 80µg/ml among all the extracts of Punica granatum fruit peels. Methanol as an extraction solvent was found to be the best in obtaining the extract of Punica granatum fruit peels rich in radical scavenging and anti-inflammatory phytoconsituents.
YEAR: 2015