This study offered a novel therapeutic strategy for improving clinical effects of acute lung injury (ALI)/acute respiratory distress syndrome and provided more evidence for the health benefits of pomegranate fruits.
RESEACH TITLE: Punicalagin exhibits negative regulatory effects on LPS-induced acute lung injury
CONDUCTED BY: Laboratory of Nutrition and Function Food, College of Light Industry Economics and Management, Jilin University, People’s Republic of China; Key Laboratory of Zoonosis, Ministry of Education College of Veterinary Medicine, Jilin University, Changchun,People’s Republic of China; Department of Chemical Engineering and Technology, College of Science and Technology, Yanbian University, Yanji, People’s Republic of China.
PUBLISHED ON: European Food Reseach and Technology
Punicalagin, mainly isolated from the fruit of Pomegranate (Punica granatum L.), is a natural polyphenolic compound. In the present study, we investigated the negative regulatory effect of punicalagin on acute lung injury (ALI) induced by Lipopolysaccharide (LPS). In the murine model of ALI, the data showed that punicalagin inhibited the production of TNF- HYPERLINK IL-1β, and IL-6 and decreased protein concentration and myeloperoxidase activity with a single 4 mg/kg dose of punicalagin prior to the administration of intratracheal LPS in the bronchoalveolar lavage fluid. Furthermore, we investigated the effects of punicalagin how to modulate signal transduction. MAPKand activation were measured by Western blot and immunocytochemical analysis. The data showed that punicalagin significantly inhibited phosphorylated p38 MAPK protein expression and shocked p65-NF- HYPERLINK translocation into the nucleus. These results indicated punicalagin may exert negative regulatory effects on ALI partly through suppressing p38 MAPKs or/and pathways. This study offered a novel therapeutic strategy for improving clinical effects of acute lung injury (ALI)/acute respiratory distress syndrome and provided more evidence for the health benefits of pomegranate fruits.