RESEARCH TITLE: Oleuropein and hydroxytyrosol protect from bisphenol A effects in livers and kidneys of lactating mother rats and their pups
COUNTRIES: Tunisia and Japan
CONDUCTED BY: Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, Tunisia; Laboratory of Biochemistry, CHU Habib Bourguiba, Tunisia; Alliance for Research on North Africa (ARENA), Graduate School of Life and Environmental Sciences, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
PUBLISHED ON: Experimental and Toxicologic Pathology
Bisphenol A (BPA) is a chemical found in hard plastics and the coatings of food and drinks cans which can behave in a similar way to estrogen and other hormones in the human body.
This study aimed to evaluate the significance of the treatment with oleuropein and hydroxytyrosol olive leaves rich extracts in reducing functional perturbations and oxidative stress arising from Bisphenol A treatment in livers and kidneys of lactating mother rats and their pups’.
For this, four groups of lactating mothers were used: controls (group A), treated with bisphenol A (group B), treated with bisphenol A and oleuropein (group C) and with bisphenol A and hydroxytyrosol (group D).
As results, we had found, in Bisphenol A treated group, either in mothers or in their pups’, a significant decrease in morphological parameters, in catalase activity and in total antioxidant capacity associated to an increase in malondialdehyde levels in livers and kidneys.
For these rats, the histological aspect showed, also, deep changes. Indeed, we had observed, in livers, hepatocellular necrosis associated to leucocytes infiltration and in kidneys tubular and glomerular necrosis.
The co-treatments with Bisphenol A and oleuropein (group C) or with Bisphenol A and hydroxytyrosol (group D) ameliorate all morphological, biochemical and histological parameters as compared to Bisphenol A treated group B. The analysis of Bisphenol A and its derivatives with LC–MS/MS showed changes in their localizations between serum, livers or kidneys in all studied groups.
In conclusion, the present study demonstrates the hepato-protective and reno-protective effects of oleuropein and hydroxytyrosol olive leaves extracts from Bisphenol A and its derivates toxicity.