In this study, we investigated the possible protective effects of Hydroxytyrosol on the oxidative and morphological alterations induced by mercury (Hg) in intact human erythrocytes.
CONDUCTED BY: British Industrial Biological Research Association
PUBLISHED ON: Food and Chemical Toxicilogy
Hydroxytyrosol (HT) is a phenolic antioxidant naturally occurring in virgin olive oil. In this study, we investigated the possible protective effects of Hydroxytyrosol on the oxidative and morphological alterations induced by mercury (Hg) in intact human erythrocytes. These cells preferentially accumulate this toxic heavy metal; more importantly, Hg-induced echinocyte formation correlates with increased coagulability of these cells. Our results indicate that Hydroxytyrosol treatment (10-50 µM) prevents the increase in hemolysis and Reactive Oxigen Species (ROS) generation induced by exposure of cells to micromolar HgCl2 concentrations as well as the decrease in GSH intracellular levels; moreover, as indicated by scanning electron microscopy, the morphological alterations are also significantly reduced by Hydroxytyrosol co-treatment. Taken together our data provide the first experimental evidence that Hydroxytyrosol has the potential to counteract mercury toxicity. The reported effect may be regarded as an additional mechanism underlying the beneficial cardio-protective effects of this dietary antioxidant, also endowed with significant anti-atherogenic and anti-inflammatory properties.