Skip to content

Pomegranate inhibits neuroinflammation and amyloidogenesis

In this study we propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer’s disease.

Hydroxytyrosol-from-extra-virgin-olive-oil
previous arrow
next arrow
Slider

RESEARCH TITLE: Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells

COUNTRIES: UK

CONDUCTED BY:  Department of Pharmacy, School of Applied Sciences, University of Huddersfield, Huddersfield, UK

 PUBLISHED ON: European Journal of Nutrition

RESEACH:

Hydroxytyrosol-from-extra-virgin-olive-oil
previous arrow
next arrow
Slider

Purpose

Pomegranate fruit, Punica granatum L. (Punicaceae), and its constituents have been shown to inhibit inflammation. In this study, we aimed to assess the effects of freeze-dried pomegranate on PGE2 production in IL-1β-stimulated SK-N-SH cells.

Methods

An enzyme immunoassay (EIA) was used to measure prostaglandin E2 (PGE2) production from supernatants of IL-1β-stimulated SK-N-SH cells. Expression of COX-2, phospho-IκB, and phospho-IKK proteins was evaluated, while NF-κB reporter gene assay was carried out in TNFα-stimulated HEK293 cells to determine the effect of freeze-dried pomegranate on NF-κB transactivation. Levels of BACE-1 and Aβ in SK-N-SH cells stimulated with IL-1β were measured with an in cell ELISA.

Hydroxytyrosol-from-extra-virgin-olive-oil
previous arrow
next arrow
Slider

Results

PWE (25–200 μg/ml) dose dependently reduced COX-2-dependent PGE2 production in SK-N-SH cells stimulated with IL-1β. Phosphorylation of IκB and IKK was significantly (p < 0.001) inhibited by freeze-dried pomegranate (50–200 μg/ml). Our studies also show that freeze-dried pomegranate (50–200 μg/ml) significantly (p < 0.01) inhibited NF-κB transactivation in TNFα-stimulated HEK293 cells. Furthermore, freeze-dried pomegranate inhibited BACE-1 and Aβ expression in SK-N-SH cells treated with IL-1β.

Conclusions

Taken together, our study demonstrates that pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer’s disease.

 YEAR: 2015

Hydroxytyrosol-from-extra-virgin-olive-oil
previous arrow
next arrow
Slider