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Enzyme-assisted supercritical fluid extraction of phenolic antioxidants from pomegranate peel

This study presents a comprehensive enzyme-assisted supercritical fluid extraction (EASCFE) process developed to extract antioxidant phenolics from Pomegranate peel. Pomegranate peel collected from local agro-processing units was treated with various commercial and feed enzyme preparations.

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The morphological changes in the ultrastructure of Pomegranate peel cell wall after enzyme treatment were visualized by field emission scanning electron microscopy (FESEM).

RESEARCH TITLE: Enzyme-assisted supercritical fluid extraction of phenolic antioxidants from pomegranate peel

COUNTRIES: USA, Pakistan, Saudi Arabia.

CONDUCTED BY: Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan; Institute of Food Science, Cornell University, USA; Department of Chemistry, Government College University, Lahore; Department of Chemistry, University of Sargodha, Pakistan; Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Saudi Arabia.

PUBLISHED ON: The Journal of Supercritical Fluids

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RESEACH:

This study presents a comprehensive enzyme-assisted supercritical fluid extraction (EASCFE) process developed to extract antioxidant phenolics from Pomegranate peel. Pomegranate peel collected from local agro-processing units was treated with various commercial and feed enzyme preparations. The morphological changes in the ultrastructure of Pomegranate peel cell wall after enzyme treatment were visualized by field emission scanning electron microscopy (FESEM).

Extraction of antioxidant phenolics from enzyme pretreated Pomegranate peel was carried out by supercritical carbon dioxide (SC-CO2) with ethanol as a co-solvent. The extracts produced were investigated for their total phenolics (TP), antioxidant activity and phenolic acids profile using HPLC-DAD-ESI-MS.

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The observed results showed that EASCFE doubled the recovery of crude extracts with increased level of phenolic constituents (TPs), and improved radical scavenging capacity (RSC), trolox equivalent antioxidant capacity (TEAC) and inhibition of linoleic acid peroxidation. HPLC-DAD-ESI-MS characterization of the extracts authenticated the presence of vanillic (108.36 μg/g of extract), ferulic (75.19 μg/g of extract) and syringic (88.24 μg/g of extract) as potent phenolic acids.

The results of this study support that EASCFE can be explored as a state-of-the-art green technological process for recovering optimum amounts of antioxidant phenolics from under-utilized pomegranate peel or other such materials. In addition, retention of appreciable level of naturally occurring vanillic, ferulic and syringic acids in the enzyme-extracted components advocates that EASCFE is a good choice for preparation of pomegranate peel extracts for food preservation as well as for the development of nutraceuticals and chemo-preventive phytomedicine.

YEAR: 2015

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