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Inhibitory effect of pomegranate on the bacterial growth of clinical isolates of pathogenic Staphylococcus aureus and Escherichia coli

In the present study major polyphenols of pomegranate arils and peel by-products were extracted in 50% (v/v) aqueous ethanol, characterized and used in microbiological assays in order to test antimicrobial activity against clinically isolated human pathogenic microorganisms.

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Data herein indicated that both pomegranate aril and peel extracts have an effective antimicrobial activity, as evidenced by the inhibitory effect on the bacterial growth of two important human pathogens, including Staphylococcus aureus and Escherichia coli, which are often involved in foodborne illness.

RESEARCH TITLE: Inhibitory effect of pomegranate (Punica granatum L.) polyphenol extracts on the bacterial growth and survival of clinical isolates of pathogenic Staphylococcus aureus and Escherichia coli

COUNTRIES: Italy

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CONDUCTED BY: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Benevento, Italy; Istituto di Scienze dell’Alimentazione-CNR, Via Roma 64, 83100 Avellino, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli “Federico II”, via S. Pansini 5, 80131 Napoli, Italy.; Fondazione SDN, Via Gianturco 113, 80143, Napoli, Italy; CEINGE-Biotecnologie Avanzate, Via G. Salvatore 486, 80145 Napoli, Italy

PUBLISHED ON: Food Chemistry

RESEACH:

Abstract

In the present study major polyphenols of pomegranate arils and peel by-products were extracted in 50% (v/v) aqueous ethanol, characterized and used in microbiological assays in order to test antimicrobial activity against clinically isolated human pathogenic microorganisms.

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Total concentration of polyphenols and in vitro antioxidant properties were determined by the Folin-Ciocalteu and DPPH methods, respectively. The most abundant bioactive molecules, including anthocyanins, catechins, tannins, gallic and ellagic acids were identified by RP-HPLC-DAD, also coupled to off-line matrix assisted laser desorption/ionization (MALDI-TOF) mass spectrometry (MS). The inhibitory spectrum of extracts against test microorganisms was assessed by the agar well-diffusion method. Data herein indicated that both pomegranate aril and peel extracts have an effective antimicrobial activity, as evidenced by the inhibitory effect on the bacterial growth of two important human pathogens, including Staphylococcus aureus and Escherichia coli, which are often involved in foodborne illness.

YEAR: 2015

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