Furthermore, another mechanism may be through anti-inflammatory and different signaling pathways, which need further investigation to elucidate this mechanism.
CONDUCTED BY: Zoology Dept., Faculty of Science, Mansoura University, Mansoura, Egypt
PUBLISHED ON: Jounal of Life Sciences
This study aimed to assess the nephroprotective effects of two pomegranate extracts, pomegranate juice and pomegranate peel methanol extract in rats with chronic renal failure (CRF) induced by adenine (AD). Thirty six male rats were allocated into six groups: Control (CO), pomegranate juice, pomegranate peel methanol extract, adenine , adenine + pomegranate juice and adenine + pomegranate peel methanol extract groups. The obtained results showed a significant increase in serum levels of creatinine (Cr), blood urea nitrogen (BUN), uric acid (UA) in AD-fed rats. In addition, relative kidney weight, urine volume and urine NAG activity were significantly increased, while creatinine clearance was decreased. A significant disturbance was observed in renal antioxidant system of AD-fed rat group represented by elevations in thiobarbituric acid reactive substance (TBARS) and protein carponyl (PC) as well as depletion in the activities of SOD and CAT. Also, a significant increases in concentration of both serum tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) accompanied by decrease in nitric oxide level were observed. Administration of pomegranate extracts, either pomegranate juice or pomegranate peel methanol extract significantly mitigated all the signs of AD-induced CRF. The results suggested that the renoprotective efficacy of pomegranate, in particular the methanol peel extract, can be attributed to antioxidant, anti-inflammatory and different signaling pathway mechanisms.
The present study suggest that pomegranate extracts, especially methanol peel extract is a potent nephroprotective agent on chronic renal failure rat model induced by adenine. This renoprotective effect of pomegranate extracts can be attributed to its high phenolic content and the mechanism of action may be through induction of various antioxidant enzymes and scavenging reactive oxygen species. Furthermore, another mechanism may be through anti-inflammatory and different signaling pathways, which need further investigation to elucidate this mechanism.